p38MAPK信号通路在猪源H9N2流感病毒导致小鼠肺损伤中作用的研究

doi:10.11843/j.issn.0366-6964.2014.02.017

Abstract

Abstract:

The aim of this study was to investigate the role of p38MAPK in acute lung injury (ALI) induced by H9N2 influenza virus isolated from swine (H9N2-SIV) in mouse model.BALB/c mice were divided into H9N2 infected group，H9N2 + p38MAPK inhibitor (SB203580) group and mock infected group.The lungs were observed for lesion of pathological change and the lung wet weight/dry weight (W/D) were investigated; The quantity of MAP kinases protein were detected by Western blot analysis; The cell count and the contents of TNF-α and IL-1β in bronchial alveolar lavaged fluid (BALF) were measured.The results were as follows：the result of western blot showed that the quantity of MAP kinases protein in lung of H9N2 group higher significantly compared with H9N2 + SB203580 group (P<0.01); Furthermore，depression，dyspnea and weight loss appeared on H9N2 infected mice markedly; Histopathologically，the similar histopathological changes such as alveolar and interstitial edema，hemorrhage，inflammation cell infiltration were also observed in lung in H9N2 + SB203580 group mice，but the degree of lung lesions was not as serious as those in H9N2 group; Moreover，the interventions of SB203580 decreased the morality of infected mice (P<0.01)，and prolonged the survival time of infected mice significantly.Meanwhile，it also decreased markedly W/D（wet weight/dry weight of lung），the number of inflammatory cell and levels of TNF-α and IL-1β in BALF (P<0.01).The results indicated that p38MAPK had an important role in the process of H9N2-SIV-induced ALI，and its inhibors SB203580 may block effectively expression of p38MAPK，and ameliorate lung injuries by inhibiting excessive discharging of TNF-α and IL-1β，which suggesting that SB203580 have benefit role to intervene the ALI induced by H9N2-SIV influenza virus in future.

CLC Number:

S855.3

WANG Cun-lian，WEI Dong，XU Ming-ju，ZHANG Rui-hua，LIU Bao-jian，WANG Guo-hua，TIAN Shu-fei，XU Tong. The Role of p38MAPK in Acute Lung Injury Induced by H9N2 Influenza Virus Isolated from Swine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(2): 281-288.

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The Role of p38MAPK in Acute Lung Injury Induced by H9N2 Influenza Virus Isolated from Swine

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